Upstream Process2018-10-19T10:04:59+01:00

Upstream Process

What is upstream processing (USP)?

The upstream part of a bioprocess refers to the initial stage in which microbes/cells are grown, e.g. bacterial or mammalian cell lines in bioreactors. Upstream processing involves all the steps related with inoculum development: Media Preparation, Cell Culture and Cell Separation & Harvest. When the cells have reached the desired density, they are harvested and moved to the downstream section of the bioprocess.

Upstream processing is usually divided into the following stages:Upstream Media Preparation

  1. Media Preparation
  2. Cell Culture
  3. Cell Separation & Cell Harvest